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Spore testing requirements
Spore testing requirements











You will need to provide us with information about what kind of sterilizers you have and how many.

spore testing requirements

Steam uses head and pressure so distribution within the chamber is not as important as it is with chemical sterilizers.Ī: If you are a new client, call us at 50. The difference is that chemical sterilizers are more sensitive to over crowding of the chamber. Q: Are steam sterilizers better than chemical sterilizers?Ī: Both steam and chemical sterilizers are effective and efficient. Exposure to sunlight will cause the tape to turn. Autoclave tape only shows heat, not pressure and certainly not chemical sterilization reagents. Q: Isn’t the indicator tape sufficient to show sterilization?Ī: No. One type of test strips for all sterilizers. The spores of both species are found on the test strips used by SMS. Oven sterilization is verified by spores of Bacillus atrophaeus, cultured at 37 C°. Steam and chemical sterilization is verified by the spores of Geobacillus stearothermophis, cultured at 55 C°. Q: Is biological monitoring (spore test strips) acceptable for all methods of sterilization?Ī: Steam, chemical and oven sterilization can all be verified by the dual species test strips used by the SMS at OHSU. In December, you will receive a hard copy of the past years record, along with your certificate of participation in the monitoring service.

Spore testing requirements verification#

If you need a copy of your record for a specific period, just call us but your email record of results is satisfactory for verification of compliance. Your records are stored in an encrypted data base on site. stearothermophis is the test species for steam and chemical sterilizer units. This reduces the chance of a false positive result to essentially zero. They are cultured at 55 C°, too hot for almost every other bacterial species.

spore testing requirements

Geobacillus stearothermophis is a thermophilic bacteria, original isolates in the thermal pools of Yellowstone. Why use two species? Bacillus atrophaeus spores are notoriously resistant to heat, making them perfect for testing heat only sterilizers like ovens. The test strips supplied by the Sterilizer Monitoring Service are imbedded with spores of two species of bacteria Geobacillus stearothermophis and Bacillus atrophaeus. Because bacterial spores are the most resistant form of bacteria, spores have been the most common test material. Biological monitoring of sterilizer function depends on the sterilizer killing the test organism.











Spore testing requirements